Product Synonym Names. Product Gene Name.
Human Eotaxin / CCL11
Ccl11 recombinant protein [ Similar Products ]. Research Use Only. For Research Use Only.
Not for use in diagnostic procedures. ModBase 3D Structure for P Greater than Sterile Filtered White lyophilized freeze-dried powder. Biological Activity. Preparation and Storage. Lyophilized Eotaxin although stable at room temperature for 3 weeks, should be stored desiccated below degree C. Upon reconstitution CCL11 should be stored at 4 degree C between days and for future use below degree C. For long term storage it is recommended to add a carrier protein 0.
Please prevent freeze-thaw cycles. Other Notes. Small volumes of Ccl11 recombinant protein vial s may occasionally become entrapped in the seal of the product vial during shipment and storage. Certain products may require to ship with dry ice and additional dry ice fee may apply. Related Product Information for Ccl11 recombinant protein.
Description: Eotaxin Mouse Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 74 amino acids and having a molecular mass of The CCL11 is purified by proprietary chromatographic techniques. CCL11 selectively recruits eosinophils by inducing their chemotaxis, and therefore, is implicated in allergic responses. The effects of CCL11 are mediated by its binding to a G-protein-linked receptor known as a chemokine receptor. The gene for human CCL11 scya11 is encoded on three exons and is located on chromosome It may not necessarily be applicable to this product.
NCBI Accession. UniProt Primary Accession. P [ Other Products ]. UniProt Related Accession. Molecular Weight.
In vitro , plasminogen was required for eosinophils to migrate through Matrigel, an artificial extracellular matrix, toward eotaxin, presumably because of a role for plasmin in degrading the basement membrane. In related studies, eotaxin autocrine signaling within eosinophils has also been suggested to promote the release of proteases required for eosinophil migration through basement membranes and the extracellular matrix We have shown that heparin was also able to indirectly protect eotaxin from elastase- and cathepsin G-mediated proteolysis by inhibiting protease action, as previously reported for IL-8 This would suggest that the high affinity interaction of eotaxin with heparin confers protection from various proteases and allows eotaxin to persist in tissues as an active chemokine.
Heparin is synthesized by mast cells and released, bound to tryptase, during mast cell degranulation A number of mast cell-derived mediators has been shown to prime or activate eosinophil responses, whereas eosinophil-derived mediators can also directly affect mast cells responses 41 - In fact, mast cell-deficient mice develop less eosinophilia than wild-type mice in models of allergic airways disease AAD Interestingly, the only other GAG found to bind eotaxin, chondroitin sulfate E, is also released from mast cells upon degranulation In a previous study, mast cells were shown to be required for eosinophil infiltration in response to eotaxin injection into air pouches of sensitized mice This could be explained by the eotaxin-heparin interaction and the protection it offers from proteolysis.
In this case the addition of heparin was not able to protect these chemokines, and, in fact, increased proteolysis. This response would be important in the control of chronic inflammation, when it would be beneficial for the neutralization of chemokines by proteolysis to reduce infiltrating leukocytes. Thus we would suggest that during chronic inflammation, mast cell tryptase may negatively regulate eosinophilia by destroying eotaxin and RANTES chemokine gradients.
Similarly, the observation reported here that heparanase cleavage of heparin greatly reduces heparin affinity for eotaxin, suggests that heparanase may also play a role in the negative regulation of eotaxin-mediated eosinophilia. Our results demonstrate that heparin did not increase murine eotaxin oligomerization. This is consistent with previous studies of human eotaxin employing mass spectrometry Recently, it has been suggested that heparin may promote heterodimerization of eotaxin with MCP-1 CCL2 59 , although the functional significance of this observation, remains to be elucidated.
MCP-1 signals through CCR2 and results in the influx of neutrophils and the induction of Th1-like inflammatory responses. These results will have important implications for understanding and directing eosinophil recruitment in various disease settings. Eosinophils have been shown to play an important role in the pathophysiology of asthma and allergic airways disease. Controlling the influx of eosinophils into the asthmatic lung has therapeutic potential. Previous studies have examined the role of heparin in controlling allergen-induced airway eosinophilia in guinea pigs 61 - Unfractionated heparin was shown to reduce rather than enhance eosinophil infiltration into the lung and bronchoalveolar lavage fluid.
These previous studies did not elucidate the mechanism responsible for the reduction in eosinophil inflammation induced by heparin but report that low molecular weight heparins and chemically modified heparins without anticoagulant activity exhibited more potent anti-inflammatory effects 62 , This suggests that the mechanism is not related to chemokine modulation, because many studies, including our current research, have shown that chemokines have a much lower affinity for low molecular weight heparins.
From the findings reported here, we would suggest that heparin helps to promote, not reduce, eosinophil recruitment, by enhancing eotaxin action through the inhibition of proteolysis and possibly the formation of a haptotactic chemokine gradient within the extracellular matrix. The potential exists for further investigation into heparin as an anticancer therapeutic to increase eosinophil homing into tumors 65 , 66 or, similarly, in the treatment of parasitic infections.
Furthermore, disruption of the heparin-eotaxin interaction, possibly through employment of heparanase or other competitive binding inhibitors, may provide therapeutic opportunities for pathologies associated with eosinophilia such as asthma and allergic airways disease. We acknowledge the technical assistance of Dr.
Shaun McColl and laboratory for technical assistance in developing the air-pouch studies, and Prof. The costs of publication of this article were defrayed in part by the payment of page charges. Section solely to indicate this fact. You'll be in good company. Journal of Lipid Research. Previous Section Next Section. View this table: In this window In a new window. Previous Section. Handel, T. CrossRef Medline Google Scholar. Kuschert, G. Hoogewerf, A. Lortat-Jacob, H. Bernfield, M. Ley, K. Wang, L. Yoshie, O. Medline Google Scholar. Rot, A.
Wiedermann, C. Middleton, J.
Eotaxin 2/CCL24 Human ELISA Kit
Parish, C. Vives, R. Webb, L. Park, P.
Sadir, R. Johnson, Z. Proudfoot, A. Jose, P. Resnick, M. Cell Mol. Kita, H. Griffiths-Johnson, D.
Human Eotaxin ELISA Kit (CCL11) (ab) | Abcam
Rothenberg, M. Ponath, P. Daugherty, B. Conroy, D. Jones, A. Freeman, C. Acta , 56 Lawman, M. Methods 69 , Mould, A. Crump, M. Martin, L. Culley, F. Ferland, C. Abraham, W. Google Scholar. He, S. Tomimori, Y.
Piliponsky, A. Oliveira, S.
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Allergy Asthma Immunol. Preparation The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin. Concentration 0. Do not freeze. It is recommended that the reagent be titrated for optimal performance. Reviews Review This Product. Related Products Description Clone Applications.
Related FAQs How many biotin molecules are per antibody structure? We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from molecules per antibody. Certificate of Analysis Lot :. Compare Data Across All Formats This data display is provided for general comparisons between formats.